Rapid screening method for compounds that affect the growth and germination of Candida albicans, using a real-time PCR thermocycler.
نویسندگان
چکیده
We propose a screening method for compounds affecting growth and germination in Candida albicans using a real-time PCR thermocycler to quantify green fluorescent protein (GFP) fluorescence. Using P(ACT1)-GFP and P(HWP1)-GFP reporter strains, the effects of a wide range of compounds on growth and hyphal formation were quantitatively assessed within 3 h after inoculation.
منابع مشابه
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 77 22 شماره
صفحات -
تاریخ انتشار 2011